primary αvβ5  (Santa Cruz Biotechnology)

Journal: OncoTargets and therapy

Article Title: The effect of antagonizing RGD-binding integrin activity in papillary thyroid cancer cell lines

doi: 10.2147/OTT.S99166

Figure Lengend Snippet: Expression of αvβ3 and αvβ5 integrins in BCPAP, K1, and TPC1 cell lines. Notes: ( A ) Immunofluorescence staining showing the distribution of αvβ3 and αvβ5 in the three cell lines. The “Merge” images correspond to the respective images situated to their left, and show the merge of DAPI staining (blue) and FITC staining (green). The 20 μm scale bar applies to all images. ( B and C ) Representative flow cytometry histograms obtained after the immunofluorescence staining of the cells. Solid lines represent the fluorescence intensity of the cells after incubation with monoclonal antibodies against αvβ3 and αvβ5 followed by incubation with the secondary antibody conjugated to FITC. Dotted lines indicate background staining with secondary antibody alone. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate.

Article Snippet: Then the cells were blocked in 5% bovine serum albumin for 40 minutes at 37°C and incubated overnight at 4°C in a solution containing diluted primary antibodies against αvβ3 (dilution 1:100) and αvβ5 (dilution 1:100) (R&D Systems).

Techniques: Expressing, Immunofluorescence, Staining, Flow Cytometry, Fluorescence, Incubation

Journal: OncoTargets and therapy

Article Title: The effect of antagonizing RGD-binding integrin activity in papillary thyroid cancer cell lines

doi: 10.2147/OTT.S99166

Figure Lengend Snippet: K1, BCPAP, and TPC1 cells detached from the flask after antagonizing αvβ3 and αvβ5. Note: Images shown at 40× magnification.

Article Snippet: Then the cells were blocked in 5% bovine serum albumin for 40 minutes at 37°C and incubated overnight at 4°C in a solution containing diluted primary antibodies against αvβ3 (dilution 1:100) and αvβ5 (dilution 1:100) (R&D Systems).

Techniques:

Journal: OncoTargets and therapy

Article Title: The effect of antagonizing RGD-binding integrin activity in papillary thyroid cancer cell lines

doi: 10.2147/OTT.S99166

Figure Lengend Snippet: The cytotoxic effect of antagonizing integrin αvβ3 and αvβ5 activity in PTC cell lines. Notes: ( A ) Cell viability after antagonizing αvβ3 and αvβ5 was assessed by CCK-8 assay. ( B ) Different degrees of cell apoptosis were induced by αvβ3 and αvβ5 antagonism in the three cell lines. The right lower quadrant of each plot contains early apoptotic cells, whereas the right upper quadrant contains late apoptotic cells. ( C ) The expression of cPARP in three PTC cell lines increased after antagonizing αvβ3 and αvβ5 activity. Abbreviations: PTC, papillary thyroid cancer; cPARP, cleaved PARP; CCK-8, cell counting kit-8; DMSO, dimethyl sulfoxide; FITC, fluorescein isothiocyanate; PE, phycoerythrin; Con, control; h, hours.

Article Snippet: Then the cells were blocked in 5% bovine serum albumin for 40 minutes at 37°C and incubated overnight at 4°C in a solution containing diluted primary antibodies against αvβ3 (dilution 1:100) and αvβ5 (dilution 1:100) (R&D Systems).

Techniques: Activity Assay, CCK-8 Assay, Expressing, Cell Counting

Journal: OncoTargets and therapy

Article Title: The effect of antagonizing RGD-binding integrin activity in papillary thyroid cancer cell lines

doi: 10.2147/OTT.S99166

Figure Lengend Snippet: Migration and invasion of all three PTc cell lines are inhibited after antagonizing αvβ3 and αvβ5 integrin activity. Notes: Data are presented as the mean ± SD of the values from three assays. in comparison with control, * P <0.05 and ** P <0.01. Abbreviations: PTc, papillary thyroid cancer; SD, standard deviation; con, control.

Article Snippet: Then the cells were blocked in 5% bovine serum albumin for 40 minutes at 37°C and incubated overnight at 4°C in a solution containing diluted primary antibodies against αvβ3 (dilution 1:100) and αvβ5 (dilution 1:100) (R&D Systems).

Techniques: Migration, Activity Assay, Comparison, Standard Deviation